Detailed information of TA system TA100860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2752210..2752847
• Replicon: chromosome
• Accession: NZ_CP028205
• Organism: Bacillus velezensis strain SRCM102741

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: C7M19_RS13485
• Protein ID: WP_003156187.1
• Coordinates: 2752497..2752847 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: C7M19_RS13480
• Protein ID: WP_003156188.1
• Coordinates: 2752210..2752491 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M19_RS13460	2748575..2749174	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
C7M19_RS13465	2749267..2749632	+	366	WP_094031521.1	holo-ACP synthase	-
C7M19_RS13470	2749797..2750804	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
C7M19_RS13475	2750921..2752090	+	1170	WP_025284272.1	alanine racemase	-
C7M19_RS13480	2752210..2752491	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
C7M19_RS13485	2752497..2752847	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
C7M19_RS13490	2752965..2753786	+	822	WP_003156182.1	STAS domain-containing protein	-
C7M19_RS13495	2753791..2754156	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
C7M19_RS13500	2754159..2754560	+	402	WP_003156178.1	anti-sigma regulatory factor	-
C7M19_RS13505	2754572..2755579	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
C7M19_RS13510	2755643..2755972	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
C7M19_RS13515	2755969..2756451	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
C7M19_RS13520	2756417..2757205	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
C7M19_RS13525	2757205..2757807	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T100860 WP_003156187.1 NZ_CP028205:2752497-2752847 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T100860 NZ_CP028205:2752497-2752847 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT100860 WP_003156188.1 NZ_CP028205:2752210-2752491 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT100860 NZ_CP028205:2752210-2752491 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5