Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 2707351..2708268 Replicon chromosome
Accession NZ_CP028204
Organism Bacillus velezensis strain SRCM102755

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag C7M18_RS13745 Protein ID WP_007407256.1
Coordinates 2707522..2708268 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag C7M18_RS13740 Protein ID WP_003154807.1
Coordinates 2707351..2707521 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7M18_RS13705 2702563..2704152 + 1590 WP_029973066.1 hypothetical protein -
C7M18_RS13710 2704165..2704590 + 426 WP_021493823.1 hypothetical protein -
C7M18_RS13715 2704595..2704792 + 198 WP_007610833.1 XkdX family protein -
C7M18_RS13720 2704849..2705610 + 762 WP_160216823.1 phage portal protein -
C7M18_RS13725 2705662..2705925 + 264 WP_021493821.1 hemolysin XhlA family protein -
C7M18_RS13730 2705939..2706202 + 264 WP_003154813.1 phage holin -
C7M18_RS13735 2706216..2707094 + 879 WP_007407257.1 N-acetylmuramoyl-L-alanine amidase -
C7M18_RS13740 2707351..2707521 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
C7M18_RS13745 2707522..2708268 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
C7M18_RS13750 2708372..2709370 - 999 WP_003154805.1 inorganic phosphate transporter -
C7M18_RS13755 2709383..2710000 - 618 WP_003154804.1 DUF47 domain-containing protein -
C7M18_RS13760 2710286..2711602 - 1317 WP_007610842.1 amino acid permease -
C7M18_RS13765 2711924..2712874 + 951 WP_160216824.1 ring-cleaving dioxygenase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-239)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T100859 WP_007407256.1 NZ_CP028204:c2708268-2707522 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T100859 NZ_CP028204:c2708268-2707522 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCACTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT100859 WP_003154807.1 NZ_CP028204:c2707521-2707351 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT100859 NZ_CP028204:c2707521-2707351 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB I2HQ15


Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References