Detailed information of TA system TA100858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1941156..1941793
• Replicon: chromosome
• Accession: NZ_CP028204
• Organism: Bacillus velezensis strain SRCM102755

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: C7M18_RS09695
• Protein ID: WP_003156187.1
• Coordinates: 1941443..1941793 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: C7M18_RS09690
• Protein ID: WP_003156188.1
• Coordinates: 1941156..1941437 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7M18_RS09670	1937521..1938120	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
C7M18_RS09675	1938213..1938578	+	366	WP_014304402.1	holo-ACP synthase	-
C7M18_RS09680	1938743..1939750	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
C7M18_RS09685	1939867..1941036	+	1170	WP_021495079.1	alanine racemase	-
C7M18_RS09690	1941156..1941437	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
C7M18_RS09695	1941443..1941793	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
C7M18_RS09700	1941911..1942732	+	822	WP_003156182.1	STAS domain-containing protein	-
C7M18_RS09705	1942737..1943102	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
C7M18_RS09710	1943105..1943506	+	402	WP_003156178.1	anti-sigma regulatory factor	-
C7M18_RS09715	1943518..1944525	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
C7M18_RS09720	1944589..1944918	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
C7M18_RS09725	1944915..1945397	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
C7M18_RS09730	1945363..1946151	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
C7M18_RS09735	1946151..1946753	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T100858 WP_003156187.1 NZ_CP028204:1941443-1941793 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T100858 NZ_CP028204:1941443-1941793 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT100858 WP_003156188.1 NZ_CP028204:1941156-1941437 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT100858 NZ_CP028204:1941156-1941437 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5