Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2488445..2489081 Replicon chromosome
Accession NZ_CP028202
Organism Bacillus subtilis strain SRCM102754

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag C7M17_RS13120 Protein ID WP_003156187.1
Coordinates 2488445..2488795 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag C7M17_RS13125 Protein ID WP_003225183.1
Coordinates 2488800..2489081 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7M17_RS13080 2483490..2484089 - 600 WP_095252677.1 phosphoserine phosphatase RsbX -
C7M17_RS13085 2484089..2484877 - 789 WP_014662857.1 RNA polymerase sigma factor SigB -
C7M17_RS13090 2484843..2485325 - 483 WP_003234299.1 anti-sigma B factor RsbW -
C7M17_RS13095 2485322..2485651 - 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
C7M17_RS13100 2485713..2486718 - 1006 Protein_2586 phosphoserine phosphatase RsbU -
C7M17_RS13105 2486730..2487131 - 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
C7M17_RS13110 2487135..2487500 - 366 WP_003225190.1 RsbT antagonist protein RsbS -
C7M17_RS13115 2487505..2488329 - 825 WP_087614324.1 RsbT co-antagonist protein RsbRA -
C7M17_RS13120 2488445..2488795 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
C7M17_RS13125 2488800..2489081 - 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
C7M17_RS13130 2489197..2490366 - 1170 WP_087614321.1 alanine racemase -
C7M17_RS13135 2490481..2491497 - 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
C7M17_RS13140 2491663..2492028 - 366 WP_015252768.1 holo-ACP synthase -
C7M17_RS13145 2492123..2492722 + 600 WP_087614319.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T100857 WP_003156187.1 NZ_CP028202:c2488795-2488445 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T100857 NZ_CP028202:c2488795-2488445 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT100857 WP_003225183.1 NZ_CP028202:c2489081-2488800 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT100857 NZ_CP028202:c2489081-2488800 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References