Detailed information of TA system TA100841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 26512..27059
• Replicon: plasmid pGMI14-004_1
• Accession: NZ_CP028195
• Organism: Escherichia coli strain CFSAN018748

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: C7A99_RS27115
• Protein ID: WP_107236369.1
• Coordinates: 26778..27059 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A2I6FMZ7
• Locus tag: C7A99_RS27110
• Protein ID: WP_000079930.1
• Coordinates: 26512..26781 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A99_RS27060	21627..21938	-	312	WP_000765906.1	hypothetical protein	-
-	22234..22295	-	62	NuclAT_0	-	-
-	22234..22295	-	62	NuclAT_0	-	-
-	22234..22295	-	62	NuclAT_0	-	-
-	22234..22295	-	62	NuclAT_0	-	-
C7A99_RS27080	23600..23740	+	141	WP_064717426.1	type I toxin-antitoxin system Hok family toxin	-
C7A99_RS27085	24020..24277	+	258	WP_000083834.1	replication regulatory protein RepA	-
C7A99_RS27095	24511..24585	+	75	WP_032319616.1	RepA leader peptide Tap	-
C7A99_RS27100	24578..25599	+	1022	Protein_34	replication initiation protein	-
C7A99_RS27110	26512..26781	+	270	WP_000079930.1	hypothetical protein	Antitoxin
C7A99_RS27115	26778..27059	+	282	WP_107236369.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C7A99_RS27745	27105..27834	+	730	Protein_37	3'-5' exonuclease	-
C7A99_RS27130	27904..28149	-	246	Protein_38	DDE-type integrase/transposase/recombinase	-
C7A99_RS27140	28559..29418	-	860	Protein_39	IS3 family transposase	-
C7A99_RS27150	29532..29928	-	397	Protein_40	ATP-binding protein	-
C7A99_RS27155	30276..30650	-	375	WP_015675362.1	heat-labile enterotoxin LT subunit B	-
C7A99_RS27160	30647..31396	-	750	WP_071782049.1	heat-labile enterotoxin LT subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	eltB / eltA	1..68950	68950
	-	inside	IScluster/Tn	-	eltB / eltA	12534..40402	27868

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10896.63 Da
• Isoelectric Point: 8.4615

>T100841 WP_107236369.1 NZ_CP028195:26778-27059 [Escherichia coli]
MMEIFWTMLASMDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T100841 NZ_CP028195:26778-27059 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCATGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2055

>AT100841 WP_000079930.1 NZ_CP028195:26512-26781 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVADK

Nucleotide

• Length: 270 bp

>AT100841 NZ_CP028195:26512-26781 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCTGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2I6FMZ7