Detailed information of TA system TA100839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 45974..46581
• Replicon: plasmid pGMI14-004_2
• Accession: NZ_CP028193
• Organism: Escherichia coli strain CFSAN018748

Toxin (Protein)

• Gene name: doc
• Uniprot ID: B7LIH8
• Locus tag: C7A99_RS26445
• Protein ID: WP_000673985.1
• Coordinates: 46195..46581 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B7LIH9
• Locus tag: C7A99_RS26440
• Protein ID: WP_000493532.1
• Coordinates: 45974..46198 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A99_RS26405	41255..41605	-	351	WP_000127482.1	addiction module antitoxin	-
C7A99_RS26410	41592..41936	-	345	WP_001146936.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C7A99_RS26415	42290..42613	-	324	WP_071702296.1	hypothetical protein	-
C7A99_RS26420	42797..43768	-	972	WP_000082158.1	IS110 family transposase	-
C7A99_RS26425	44336..44995	+	660	WP_000516232.1	peptidyl-arginine deiminase	-
C7A99_RS26430	45085..45387	+	303	WP_001096511.1	hypothetical protein	-
C7A99_RS26440	45974..46198	+	225	WP_000493532.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C7A99_RS26445	46195..46581	+	387	WP_000673985.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C7A99_RS26455	47735..48592	-	858	WP_000130985.1	incFII family plasmid replication initiator RepA	-
C7A99_RS26460	48585..48659	-	75	WP_001365705.1	RepA leader peptide Tap	-
C7A99_RS26470	48904..49152	-	249	WP_000083839.1	replication regulatory protein RepA	-
C7A99_RS26475	49392..49982	-	591	WP_000766804.1	DUF2726 domain-containing protein	-
C7A99_RS26480	50020..50229	-	210	WP_001298565.1	hemolysin expression modulator Hha	-
C7A99_RS26485	50275..50736	-	462	WP_001233837.1	thermonuclease family protein	-
C7A99_RS26490	50981..51172	-	192	WP_071602422.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..52918	52918
	-	flank	IS/Tn	-	-	42797..43768	971

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (11-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-52)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14370.62 Da
• Isoelectric Point: 7.0732

>T100839 WP_000673985.1 NZ_CP028193:46195-46581 [Escherichia coli]
MKFVSSQEVIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYHYEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVA
QVFLKRNGVHIISSRISFDEMQIIALNAATGEYNWKMVSDHLKAIILN

Nucleotide

• Length: 387 bp

>T100839 NZ_CP028193:46195-46581 [Escherichia coli]
ATGAAATTCGTTAGTTCACAGGAAGTGATTGAGTTTCATGACCGCTTGATTTCGCGTGATGGTGGCGTGGCGGGTATGCC
AGAACCCGGTCGCGCTGACGCGATCATTCATCGTGTTTTGAACATGTATCATTATGAAGGTGTGACAGATATTATGGATC
TGGCTGCCGTGTATTTAGTTGCTATTGCTCGTGGTCACATTTTCAACGATGCTAATAAGCGAACAGCCTTGTTTGTTGCA
CAGGTTTTTTTAAAACGGAATGGTGTACATATTATTAGTTCACGTATTTCGTTTGATGAAATGCAAATTATCGCTTTGAA
TGCTGCAACAGGAGAATATAACTGGAAGATGGTTTCTGACCATCTTAAAGCCATTATTCTAAATTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8361.39 Da
• Isoelectric Point: 5.6713

>AT100839 WP_000493532.1 NZ_CP028193:45974-46198 [Escherichia coli]
MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDAFERYRKAALDAEFAEIMQSHGTAYTELSKR

Nucleotide

• Length: 225 bp

>AT100839 NZ_CP028193:45974-46198 [Escherichia coli]
ATGTTTACATGTAACTATAGCCATGCTCGCGATAACCTAGCCTCGTTGCTCGAGCTGGCGGTTGATGAAAATCAGCCTGT
TGAGATTCAGCGCCGTAAAGGTGGCGCGGCTGTACTGGTTGATAAAGACGCTTTTGAACGATACCGTAAAGCGGCTCTGG
ATGCTGAGTTTGCTGAGATCATGCAAAGTCATGGGACGGCCTATACGGAGCTTTCTAAGCGATGA

Structures

Toxin

• AlphaFold DB: A0A086V9Y9

Antitoxin

• AlphaFold DB: A0A086V9Y8