100838

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 38770..39263 Replicon plasmid pGMI14-004_2
Accession NZ_CP028193
Organism Escherichia coli strain CFSAN018748

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag C7A99_RS26385 Protein ID WP_001230709.1
Coordinates 39009..39263 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag C7A99_RS26380 Protein ID WP_000151456.1
Coordinates 38770..39012 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A99_RS26340 34023..34625 + 603 WP_077638166.1 transglycosylase SLT domain-containing protein -
C7A99_RS26345 34922..35743 - 822 WP_016235962.1 DUF945 domain-containing protein -
C7A99_RS26350 35854..36039 - 186 Protein_38 hypothetical protein -
C7A99_RS26355 36275..36577 - 303 WP_000379301.1 hypothetical protein -
C7A99_RS26360 36653..36886 - 234 WP_000093744.1 hypothetical protein -
C7A99_RS26365 37314..37601 - 288 WP_000606467.1 hypothetical protein -
C7A99_RS26370 37906..38199 - 294 WP_000673428.1 hypothetical protein -
C7A99_RS26380 38770..39012 - 243 WP_000151456.1 hypothetical protein Antitoxin
C7A99_RS26385 39009..39263 - 255 WP_001230709.1 hypothetical protein Toxin
C7A99_RS26390 39260..39802 - 543 WP_001083904.1 hypothetical protein -
C7A99_RS26395 39906..40421 - 516 WP_001025393.1 J domain-containing protein -
C7A99_RS26405 41255..41605 - 351 WP_000127482.1 addiction module antitoxin -
C7A99_RS26410 41592..41936 - 345 WP_001146936.1 type II toxin-antitoxin system RelE/ParE family toxin -
C7A99_RS26415 42290..42613 - 324 WP_071702296.1 hypothetical protein -
C7A99_RS26420 42797..43768 - 972 WP_000082158.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..52918 52918
- flank IS/Tn - - 42797..43768 971


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Antitoxin MqsA/HigA-2

(1-131)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T100838 WP_001230709.1 NZ_CP028193:c39263-39009 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T100838 NZ_CP028193:c39263-39009 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT100838 WP_000151456.1 NZ_CP028193:c39012-38770 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT100838 NZ_CP028193:c39012-38770 [Escherichia coli]
ATGACTATCGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References