Detailed information of TA system TA100836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4864484..4865079
• Replicon: chromosome
• Accession: NZ_CP028192
• Organism: Escherichia coli strain CFSAN018748

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: C7A99_RS25070
• Protein ID: WP_000239581.1
• Coordinates: 4864484..4864834 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: C7A99_RS25075
• Protein ID: WP_001223213.1
• Coordinates: 4864828..4865079 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A99_RS25045	4859858..4860880	-	1023	WP_001298067.1	ABC transporter permease	-
C7A99_RS25050	4860894..4862396	-	1503	WP_107236277.1	sugar ABC transporter ATP-binding protein	-
C7A99_RS25060	4862609..4863565	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
C7A99_RS25065	4863875..4864405	+	531	WP_000055075.1	inorganic diphosphatase	-
C7A99_RS25070	4864484..4864834	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
C7A99_RS25075	4864828..4865079	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
C7A99_RS25080	4865291..4865632	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
C7A99_RS25085	4865635..4869414	-	3780	WP_057695427.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T100836 WP_000239581.1 NZ_CP028192:c4864834-4864484 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T100836 NZ_CP028192:c4864834-4864484 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT100836 WP_001223213.1 NZ_CP028192:c4865079-4864828 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT100836 NZ_CP028192:c4865079-4864828 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAATAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTAACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7