Detailed information of TA system TA100834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4631627..4632051
• Replicon: chromosome
• Accession: NZ_CP028192
• Organism: Escherichia coli strain CFSAN018748

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: C7A99_RS27720
• Protein ID: WP_001306201.1
• Coordinates: 4631627..4631815 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: C7A99_RS23885
• Protein ID: WP_000125566.1
• Coordinates: 4631818..4632051 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A99_RS23855	4627189..4628475	+	1287	WP_000800470.1	peptidylprolyl isomerase SurA	-
C7A99_RS23860	4628475..4629464	+	990	WP_000241280.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
C7A99_RS23865	4629461..4630282	+	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
C7A99_RS23870	4630285..4630662	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
C7A99_RS23875	4630669..4631517	+	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
C7A99_RS27715	4631514..4631630	-	117	WP_000148634.1	CcdB family protein	-
C7A99_RS27720	4631627..4631815	-	189	WP_001306201.1	CcdB family protein	Toxin
C7A99_RS23885	4631818..4632051	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
C7A99_RS23890	4632137..4632616	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
C7A99_RS23895	4632808..4634670	-	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
C7A99_RS23900	4634663..4635193	-	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
C7A99_RS23905	4635301..4636632	-	1332	WP_001183198.1	MFS transporter	-
C7A99_RS23910	4636691..4636978	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-57)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7167.44 Da
• Isoelectric Point: 11.6113

>T100834 WP_001306201.1 NZ_CP028192:c4631815-4631627 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGS

Nucleotide

• Length: 189 bp

>T100834 NZ_CP028192:c4631815-4631627 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGGTTTATCCGTATTCGCCCTCCAGAACGGCTTAACA
CAATCCTTTTACTGGTAGACGGCTCATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT100834 WP_000125566.1 NZ_CP028192:c4632051-4631818 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT100834 NZ_CP028192:c4632051-4631818 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9