Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4168815..4169433 Replicon chromosome
Accession NZ_CP028192
Organism Escherichia coli strain CFSAN018748

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C7A99_RS21560 Protein ID WP_001291435.1
Coordinates 4169215..4169433 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C7A99_RS21555 Protein ID WP_000344800.1
Coordinates 4168815..4169189 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A99_RS21545 4163904..4165097 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C7A99_RS21550 4165120..4168269 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
C7A99_RS21555 4168815..4169189 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C7A99_RS21560 4169215..4169433 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C7A99_RS21565 4169605..4170156 + 552 WP_000102564.1 maltose O-acetyltransferase -
C7A99_RS21570 4170272..4170742 + 471 WP_063085325.1 YlaC family protein -
C7A99_RS21575 4170906..4172456 + 1551 WP_063085326.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C7A99_RS21580 4172498..4172840 - 343 Protein_3933 DUF1428 family protein -
C7A99_RS21590 4173219..4173530 + 312 WP_000409911.1 MGMT family protein -
C7A99_RS21595 4173561..4174133 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T100831 WP_001291435.1 NZ_CP028192:4169215-4169433 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T100831 NZ_CP028192:4169215-4169433 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT100831 WP_000344800.1 NZ_CP028192:4168815-4169189 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT100831 NZ_CP028192:4168815-4169189 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References