Detailed information of TA system TA100830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3700797..3701502
• Replicon: chromosome
• Accession: NZ_CP028192
• Organism: Escherichia coli strain CFSAN018748

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C7A99_RS19185
• Protein ID: WP_107236063.1
• Coordinates: 3700797..3701183 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C7A99_RS19190
• Protein ID: WP_001280945.1
• Coordinates: 3701173..3701502 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A99_RS19165	3696801..3697427	+	627	WP_001314584.1	glutathione S-transferase GstB	-
C7A99_RS19170	3697424..3698539	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
C7A99_RS19175	3698650..3699033	-	384	WP_000497137.1	biofilm formation regulator BssR	-
C7A99_RS19180	3699246..3700571	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
C7A99_RS19185	3700797..3701183	+	387	WP_107236063.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C7A99_RS19190	3701173..3701502	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
C7A99_RS19195	3701653..3702693	+	1041	WP_107236064.1	IS481 family transposase	-
C7A99_RS19200	3702780..3704108	-	1329	WP_107236065.1	GGDEF domain-containing protein	-
C7A99_RS19205	3704116..3706464	-	2349	WP_021523051.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3701653..3702693	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14259.43 Da
• Isoelectric Point: 9.9296

>T100830 WP_107236063.1 NZ_CP028192:3700797-3701183 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLALSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T100830 NZ_CP028192:3700797-3701183 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
GAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT100830 WP_001280945.1 NZ_CP028192:3701173-3701502 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT100830 NZ_CP028192:3701173-3701502 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA