Detailed information of TA system TA100829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3109337..3109975
• Replicon: chromosome
• Accession: NZ_CP028192
• Organism: Escherichia coli strain CFSAN018748

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: C7A99_RS16160
• Protein ID: WP_000813797.1
• Coordinates: 3109799..3109975 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C7A99_RS16155
• Protein ID: WP_001270286.1
• Coordinates: 3109337..3109753 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A99_RS16135	3104489..3105430	-	942	WP_107235969.1	ABC transporter permease	-
C7A99_RS16140	3105431..3106444	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
C7A99_RS16145	3106462..3107607	-	1146	WP_107235970.1	ABC transporter substrate-binding protein	-
C7A99_RS16150	3107852..3109258	-	1407	WP_059341544.1	PLP-dependent aminotransferase family protein	-
C7A99_RS16155	3109337..3109753	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C7A99_RS16160	3109799..3109975	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C7A99_RS16165	3110197..3110427	+	231	WP_000494244.1	YncJ family protein	-
C7A99_RS16170	3110519..3112480	-	1962	WP_063085534.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C7A99_RS16175	3112553..3113089	-	537	WP_000429507.1	DNA-binding transcriptional regulator SutR	-
C7A99_RS16180	3113142..3114353	+	1212	WP_107235971.1	BenE family transporter YdcO	-
C7A99_RS16185	3114461..3114919	-	459	WP_000526135.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3114461..3114919	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T100829 WP_000813797.1 NZ_CP028192:c3109975-3109799 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T100829 NZ_CP028192:c3109975-3109799 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT100829 WP_001270286.1 NZ_CP028192:c3109753-3109337 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT100829 NZ_CP028192:c3109753-3109337 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A829J715