Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1404155..1404809 Replicon chromosome
Accession NZ_CP028192
Organism Escherichia coli strain CFSAN018748

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag C7A99_RS07255 Protein ID WP_000244781.1
Coordinates 1404402..1404809 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C7A99_RS07250 Protein ID WP_000354046.1
Coordinates 1404155..1404421 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A99_RS07230 1400243..1401676 - 1434 WP_032281563.1 6-phospho-beta-glucosidase BglA -
C7A99_RS07235 1401721..1402032 + 312 WP_001182952.1 N(4)-acetylcytidine aminohydrolase -
C7A99_RS07240 1402196..1402855 + 660 WP_000250269.1 hemolysin III family protein -
C7A99_RS07245 1402932..1403912 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
C7A99_RS07250 1404155..1404421 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C7A99_RS07255 1404402..1404809 + 408 WP_000244781.1 protein YgfX Toxin
C7A99_RS07260 1404849..1405370 - 522 WP_001055888.1 flavodoxin FldB -
C7A99_RS07265 1405482..1406378 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
C7A99_RS07270 1406403..1407113 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C7A99_RS07275 1407119..1408852 + 1734 WP_107235651.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T100818 WP_000244781.1 NZ_CP028192:1404402-1404809 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T100818 NZ_CP028192:1404402-1404809 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT100818 WP_000354046.1 NZ_CP028192:1404155-1404421 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT100818 NZ_CP028192:1404155-1404421 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References