Detailed information of TA system TA100783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 22366..23284
• Replicon: plasmid pGMI16-001
• Accession: NZ_CP028188
• Organism: Campylobacter coli strain CFSAN054106

Toxin (Protein)

• Gene name: Fic
• Uniprot ID: A0A5T1VVN4
• Locus tag: C9J81_RS10240
• Protein ID: WP_045243721.1
• Coordinates: 22366..23049 (-)
• Length: 228 a.a.

Antitoxin (Protein)

• Gene name: Fti
• Uniprot ID: A0A0Q2TTN4
• Locus tag: C9J81_RS10245
• Protein ID: WP_002804094.1
• Coordinates: 23042..23284 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9J81_RS10205	17686..18069	+	384	WP_002797386.1	DNA transfer protein	-
C9J81_RS10210	18032..20188	+	2157	WP_107128236.1	relaxase/mobilization nuclease domain-containing protein	-
C9J81_RS10220	20429..20653	-	225	WP_107128237.1	hypothetical protein	-
C9J81_RS10225	20656..21342	-	687	WP_002782958.1	AAA family ATPase	-
C9J81_RS10230	21434..21829	-	396	WP_107128238.1	conjugal transfer protein TraM	-
C9J81_RS10235	21894..22361	-	468	WP_107128239.1	signal peptidase I	-
C9J81_RS10240	22366..23049	-	684	WP_045243721.1	Fic family protein	Toxin
C9J81_RS10245	23042..23284	-	243	WP_002804094.1	hypothetical protein	Antitoxin
C9J81_RS10250	23375..24094	-	720	WP_002784845.1	conjugal transfer protein TraL	-
C9J81_RS10255	24111..25934	-	1824	WP_107128240.1	type IV secretory system conjugative DNA transfer family protein	-
C9J81_RS10260	25931..28141	-	2211	WP_107128241.1	DUF1738 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..30303	30303

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (60-169)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 228 a.a.
• Molecular weight: 26041.28 Da
• Isoelectric Point: 10.1734

>T100783 WP_045243721.1 NZ_CP028188:c23049-22366 [Campylobacter coli]
MIDKVLKFLDDGIPKNDFDRQLLASNLVGATNLIELNKKERIITLQAAKKLELNPIKGNFDYQHLKDIHKALFKDVYTWA
GQDRMQMGLKEKFAKYAPNGAIINFVPGKELDKYSKIIFDELAKNNYLKNSKDLNHFAKNLAKFMGEINALHPFREGNGR
TQRIFLNELAKNAGYKLDLNLISKHKMIHACVEASQLKPGRLEALIKDNLKNFRQNLDLEQNKGMSL

Nucleotide

• Length: 684 bp

>T100783 NZ_CP028188:c23049-22366 [Campylobacter coli]
ATGATTGATAAAGTTTTGAAATTTTTAGATGATGGTATTCCTAAAAATGATTTTGATAGACAACTTTTAGCTTCTAATTT
AGTAGGCGCAACAAATTTAATAGAGTTAAATAAAAAAGAAAGAATAATAACTCTACAAGCCGCTAAAAAATTAGAGCTTA
ATCCAATTAAAGGCAATTTTGACTATCAACACTTAAAAGATATACATAAAGCACTTTTTAAAGATGTTTATACTTGGGCG
GGACAAGATAGAATGCAAATGGGGCTAAAAGAAAAATTTGCAAAATATGCACCCAATGGAGCAATTATTAATTTTGTTCC
TGGAAAAGAGTTAGATAAATACTCTAAAATAATTTTTGATGAACTTGCAAAAAATAATTATCTAAAAAACTCCAAAGATC
TAAATCATTTTGCAAAAAATTTAGCAAAATTTATGGGCGAAATCAATGCTTTACACCCTTTTAGAGAAGGTAACGGCAGA
ACTCAAAGAATATTTTTAAATGAATTAGCTAAAAATGCAGGTTATAAACTTGATTTAAATTTAATTTCTAAGCATAAAAT
GATACATGCTTGCGTAGAAGCTTCACAATTAAAACCTGGAAGATTGGAGGCTTTAATTAAAGACAATCTAAAAAACTTTA
GACAAAATTTAGATTTGGAGCAAAATAAAGGAATGTCGTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9452.68 Da
• Isoelectric Point: 4.3535

>AT100783 WP_002804094.1 NZ_CP028188:c23284-23042 [Campylobacter coli]
MQKQYPEVHSLEESLAILKKYKDDLTKEQYEQNKSIICGFAIENMFANEEDIINLIKVDKQEKTPDEIIAEYKKEWGVND

Nucleotide

• Length: 243 bp

>AT100783 NZ_CP028188:c23284-23042 [Campylobacter coli]
ATGCAAAAACAATATCCCGAAGTGCATAGCTTAGAAGAAAGCCTTGCAATACTTAAAAAATATAAAGATGATTTAACCAA
AGAGCAATATGAGCAAAATAAATCTATTATTTGTGGATTTGCTATTGAGAATATGTTTGCAAATGAAGAAGACATTATAA
ATCTCATAAAAGTAGATAAACAAGAAAAAACACCTGATGAAATAATCGCCGAATATAAAAAAGAGTGGGGTGTAAATGAT
TGA

Structures

Toxin

• AlphaFold DB: A0A5T1VVN4

Antitoxin

• AlphaFold DB: A0A0Q2TTN4