Detailed information of TA system TA100781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 34298..34941
• Replicon: plasmid pGMI16-006_1
• Accession: NZ_CP028177
• Organism: Klebsiella pneumoniae strain CFSAN054111

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: C9J89_RS26615
• Protein ID: WP_107240835.1
• Coordinates: 34525..34941 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: R4IT22
• Locus tag: C9J89_RS26610
• Protein ID: WP_016338373.1
• Coordinates: 34298..34528 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9J89_RS26565	29496..29969	+	474	WP_004152341.1	YkgJ family cysteine cluster protein	-
C9J89_RS26575	31183..31965	-	783	WP_004152340.1	site-specific integrase	-
C9J89_RS26580	31965..32297	-	333	WP_004152339.1	hypothetical protein	-
C9J89_RS26585	32304..32702	-	399	WP_004171440.1	hypothetical protein	-
C9J89_RS26590	32728..33057	-	330	WP_004152337.1	hypothetical protein	-
C9J89_RS26595	33085..33393	-	309	WP_004152336.1	hypothetical protein	-
C9J89_RS28615	33880..34341	-	462	WP_199852547.1	hypothetical protein	-
C9J89_RS26610	34298..34528	+	231	WP_016338373.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C9J89_RS26615	34525..34941	+	417	WP_107240835.1	type II toxin-antitoxin system VapC family toxin	Toxin
C9J89_RS26620	34982..35859	-	878	Protein_40	restriction endonuclease	-
C9J89_RS26625	36518..37795	+	1278	WP_016338369.1	HlyD family secretion protein	-
C9J89_RS26630	37785..39893	+	2109	WP_016338368.1	peptidase domain-containing ABC transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(D) / blaTEM-1B / aac(3)-IId / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaSHV-12	-	1..116768	116768
	-	flank	IS/Tn	-	-	28108..29376	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15040.54 Da
• Isoelectric Point: 9.2957

>T100781 WP_107240835.1 NZ_CP028177:34525-34941 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRFAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T100781 NZ_CP028177:34525-34941 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGCGCC
GCGGTGGATGCCACGACGGACATCCGGGTGGCGCTGCGCTTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCGGCGCAGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8751.77 Da
• Isoelectric Point: 4.3533

>AT100781 WP_016338373.1 NZ_CP028177:34298-34528 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT100781 NZ_CP028177:34298-34528 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGAGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A5Q9LN61