Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1026064..1026683 Replicon chromosome
Accession NZ_CP028176
Organism Klebsiella pneumoniae strain CFSAN054111

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag C9J89_RS05210 Protein ID WP_002892050.1
Coordinates 1026465..1026683 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag C9J89_RS05205 Protein ID WP_002892066.1
Coordinates 1026064..1026438 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9J89_RS05195 1021216..1022409 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C9J89_RS05200 1022432..1025578 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
C9J89_RS05205 1026064..1026438 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
C9J89_RS05210 1026465..1026683 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
C9J89_RS05215 1026842..1027408 + 567 WP_002892030.1 maltose O-acetyltransferase -
C9J89_RS28250 1027380..1027520 - 141 WP_004147370.1 hypothetical protein -
C9J89_RS05220 1027541..1028011 + 471 WP_002892026.1 YlaC family protein -
C9J89_RS05225 1027986..1029437 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
C9J89_RS05230 1029538..1030236 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
C9J89_RS05235 1030233..1030373 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
C9J89_RS05240 1030373..1030636 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T100764 WP_002892050.1 NZ_CP028176:1026465-1026683 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T100764 NZ_CP028176:1026465-1026683 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT100764 WP_002892066.1 NZ_CP028176:1026064-1026438 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT100764 NZ_CP028176:1026064-1026438 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References