Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 107616..108163 Replicon plasmid pGMI17-004_1
Accession NZ_CP028168
Organism Escherichia coli strain CFSAN064036

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag C6668_RS26000 Protein ID WP_000969996.1
Coordinates 107616..107897 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag C6668_RS26005 Protein ID WP_000079928.1
Coordinates 107894..108163 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6668_RS25970 102627..102869 + 243 WP_000844627.1 transposase -
C6668_RS25975 102901..103551 - 651 WP_000164043.1 tetracycline resistance transcriptional repressor TetR(A) -
C6668_RS25980 103657..104856 + 1200 WP_000804064.1 tetracycline efflux MFS transporter Tet(A) -
C6668_RS25985 104888..105748 - 861 WP_000493383.1 EamA family transporter -
C6668_RS25990 105757..106371 - 615 Protein_119 helix-turn-helix domain-containing protein -
C6668_RS26475 106333..106491 + 159 WP_197711125.1 MerR family transcriptional regulator -
C6668_RS25995 106728..107576 - 849 WP_001057996.1 3'-5' exonuclease -
C6668_RS26000 107616..107897 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6668_RS26005 107894..108163 - 270 WP_000079928.1 hypothetical protein Antitoxin
C6668_RS26020 109072..110103 - 1032 WP_000907875.1 replication initiation protein -
C6668_RS26035 111076..111339 + 264 WP_001324596.1 ash family protein -
C6668_RS26040 111257..111544 + 288 WP_000074849.1 conjugal transfer protein TraA -
C6668_RS26050 111986..112519 + 534 WP_001303319.1 transcription termination factor NusG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / sul1 / qacE / ant(3'')-Ia / dfrA1 / aph(6)-Id / aph(3'')-Ib / sul2 / tet(A) - 1..119076 119076


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T100759 WP_000969996.1 NZ_CP028168:c107897-107616 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T100759 NZ_CP028168:c107897-107616 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT100759 WP_000079928.1 NZ_CP028168:c108163-107894 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT100759 NZ_CP028168:c108163-107894 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References