Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 8970..9562 Replicon plasmid pGMI17-004_2
Accession NZ_CP028167
Organism Escherichia coli strain CFSAN064036

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag C6668_RS25120 Protein ID WP_000520549.1
Coordinates 8970..9218 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag C6668_RS25125 Protein ID WP_000681613.1
Coordinates 9215..9562 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6668_RS26335 5846..6361 + 516 WP_001025395.1 J domain-containing protein -
C6668_RS25090 6466..7008 + 543 WP_001083906.1 hypothetical protein -
C6668_RS25095 7005..7256 + 252 WP_001230707.1 hypothetical protein -
C6668_RS25100 7243..7470 + 228 WP_000801440.1 hypothetical protein -
C6668_RS25110 7925..8224 + 300 WP_000650309.1 hypothetical protein -
C6668_RS25115 8315..8665 + 351 WP_000854259.1 hypothetical protein -
C6668_RS25120 8970..9218 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
C6668_RS25125 9215..9562 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6668_RS25135 9923..10426 + 504 WP_000781813.1 hypothetical protein -
C6668_RS25140 10430..11659 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
C6668_RS25145 11736..12029 + 294 WP_060598542.1 LuxR family transcriptional regulator -
C6668_RS25150 12026..12241 + 216 WP_000760375.1 EexN family lipoprotein -
C6668_RS25155 12597..13216 + 620 Protein_17 lytic transglycosylase domain-containing protein -
C6668_RS25160 13284..13556 + 273 WP_001328551.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..33305 33305


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T100754 WP_000520549.1 NZ_CP028167:8970-9218 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T100754 NZ_CP028167:8970-9218 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT100754 WP_000681613.1 NZ_CP028167:9215-9562 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT100754 NZ_CP028167:9215-9562 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References