100753

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 7005..7470 Replicon plasmid pGMI17-004_2
Accession NZ_CP028167
Organism Escherichia coli strain CFSAN064036

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag C6668_RS25095 Protein ID WP_001230707.1
Coordinates 7005..7256 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag C6668_RS25100 Protein ID WP_000801440.1
Coordinates 7243..7470 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6668_RS25070 2486..3013 - 528 WP_000577043.1 DUF2726 domain-containing protein -
C6668_RS25075 3148..3552 - 405 WP_012421203.1 hypothetical protein -
C6668_RS25080 3565..4431 - 867 WP_001353743.1 replication initiation protein -
C6668_RS26335 5846..6361 + 516 WP_001025395.1 J domain-containing protein -
C6668_RS25090 6466..7008 + 543 WP_001083906.1 hypothetical protein -
C6668_RS25095 7005..7256 + 252 WP_001230707.1 hypothetical protein Toxin
C6668_RS25100 7243..7470 + 228 WP_000801440.1 hypothetical protein Antitoxin
C6668_RS25110 7925..8224 + 300 WP_000650309.1 hypothetical protein -
C6668_RS25115 8315..8665 + 351 WP_000854259.1 hypothetical protein -
C6668_RS25120 8970..9218 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
C6668_RS25125 9215..9562 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
C6668_RS25135 9923..10426 + 504 WP_000781813.1 hypothetical protein -
C6668_RS25140 10430..11659 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
C6668_RS25145 11736..12029 + 294 WP_060598542.1 LuxR family transcriptional regulator -
C6668_RS25150 12026..12241 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..33305 33305


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T100753 WP_001230707.1 NZ_CP028167:7005-7256 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T100753 NZ_CP028167:7005-7256 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT100753 WP_000801440.1 NZ_CP028167:7243-7470 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT100753 NZ_CP028167:7243-7470 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References