Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4416661..4417279 Replicon chromosome
Accession NZ_CP028166
Organism Escherichia coli strain CFSAN064036

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C6668_RS23040 Protein ID WP_001291435.1
Coordinates 4416661..4416879 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C6668_RS23045 Protein ID WP_000344800.1
Coordinates 4416905..4417279 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6668_RS23005 4411950..4412522 + 573 WP_000779831.1 YbaY family lipoprotein -
C6668_RS23010 4412553..4412864 - 312 WP_000409911.1 MGMT family protein -
C6668_RS23020 4413243..4413596 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C6668_RS23025 4413638..4415188 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C6668_RS23030 4415352..4415822 - 471 WP_000136192.1 YlaC family protein -
C6668_RS23035 4415938..4416489 - 552 WP_000102564.1 maltose O-acetyltransferase -
C6668_RS23040 4416661..4416879 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C6668_RS23045 4416905..4417279 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C6668_RS23050 4417825..4420974 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
C6668_RS23055 4420997..4422190 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T100752 WP_001291435.1 NZ_CP028166:c4416879-4416661 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T100752 NZ_CP028166:c4416879-4416661 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT100752 WP_000344800.1 NZ_CP028166:c4417279-4416905 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT100752 NZ_CP028166:c4417279-4416905 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References