Detailed information of TA system TA100751

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 4243835..4244514
• Replicon: chromosome
• Accession: NZ_CP028166
• Organism: Escherichia coli strain CFSAN064036

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: C6668_RS22105
• Protein ID: WP_000854680.1
• Coordinates: 4243835..4244176 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: C6668_RS22110
• Protein ID: WP_000070396.1
• Coordinates: 4244197..4244514 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6668_RS22080	4239112..4239513	+	402	WP_000174677.1	sigma factor-binding protein Crl	-
C6668_RS22085	4239552..4240607	-	1056	WP_000749863.1	phosphoporin PhoE	-
C6668_RS22090	4240895..4241998	+	1104	WP_001285288.1	glutamate 5-kinase	-
C6668_RS22095	4242010..4243263	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
C6668_RS22105	4243835..4244176	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
C6668_RS22110	4244197..4244514	-	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
C6668_RS22115	4244533..4244754	-	222	WP_000691994.1	DUF987 domain-containing protein	-
C6668_RS22120	4244763..4245239	-	477	WP_000811693.1	RadC family protein	-
C6668_RS22125	4245255..4245713	-	459	WP_000211838.1	antirestriction protein	-
C6668_RS22130	4245811..4246050	-	240	Protein_4054	DUF905 domain-containing protein	-
C6668_RS22135	4246127..4246594	-	468	WP_001385283.1	hypothetical protein	-
C6668_RS22140	4246617..4247060	-	444	WP_000649865.1	hypothetical protein	-
C6668_RS22145	4247060..4247296	-	237	WP_001144031.1	hypothetical protein	-
C6668_RS22150	4247337..4248038	-	702	WP_047649085.1	WYL domain-containing protein	-
C6668_RS22155	4248255..4249076	-	822	WP_023565123.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T100751 WP_000854680.1 NZ_CP028166:c4244176-4243835 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T100751 NZ_CP028166:c4244176-4243835 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT100751 WP_000070396.1 NZ_CP028166:c4244514-4244197 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT100751 NZ_CP028166:c4244514-4244197 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7