Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3653518..3654015 Replicon chromosome
Accession NZ_CP028166
Organism Escherichia coli strain CFSAN064036

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C6668_RS19080 Protein ID WP_001173343.1
Coordinates 3653842..3654015 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag C6668_RS19075 Protein ID WP_000398619.1
Coordinates 3653518..3653814 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6668_RS19050 3650249..3650968 - 720 WP_000611288.1 two-component system response regulator DcuR -
C6668_RS19055 3650965..3652596 - 1632 WP_001216477.1 sensor histidine kinase -
C6668_RS19060 3652777..3653007 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
C6668_RS19065 3653019..3653291 + 273 WP_000405651.1 N-acetyltransferase -
C6668_RS19075 3653518..3653814 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C6668_RS19080 3653842..3654015 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C6668_RS19085 3654134..3655651 - 1518 WP_001295074.1 lysine--tRNA ligase -
C6668_RS19090 3655888..3657345 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T100748 WP_001173343.1 NZ_CP028166:3653842-3654015 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T100748 NZ_CP028166:3653842-3654015 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT100748 WP_000398619.1 NZ_CP028166:3653518-3653814 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT100748 NZ_CP028166:3653518-3653814 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References