Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 821682..822208 Replicon chromosome
Accession NZ_CP028166
Organism Escherichia coli strain CFSAN064036

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag C6668_RS04460 Protein ID WP_000323025.1
Coordinates 821682..821969 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag C6668_RS04465 Protein ID WP_000534858.1
Coordinates 821969..822208 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6668_RS04425 817511..818323 - 813 Protein_826 DUF523 and DUF1722 domain-containing protein -
C6668_RS04430 818516..819040 + 525 WP_000780584.1 lipocalin family protein -
C6668_RS04435 819196..819573 - 378 WP_001204787.1 antitermination protein -
C6668_RS04440 819591..820640 - 1050 WP_032187401.1 DUF968 domain-containing protein -
C6668_RS04445 820642..820920 - 279 WP_001515373.1 hypothetical protein -
C6668_RS04450 820987..821238 - 252 WP_001463433.1 hypothetical protein -
C6668_RS04455 821455..821610 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
C6668_RS04460 821682..821969 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C6668_RS04465 821969..822208 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C6668_RS04470 822233..822538 + 306 WP_072134164.1 hypothetical protein -
C6668_RS04475 822741..823073 + 333 WP_001301033.1 protein FlxA -
C6668_RS04485 823510..824850 - 1341 WP_000589013.1 ISNCY family transposase -
C6668_RS26360 825619..825904 - 286 Protein_838 hypothetical protein -
C6668_RS26365 826070..826252 - 183 Protein_839 hypothetical protein -
C6668_RS04505 826515..826871 - 357 WP_001315552.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 784148..835348 51200


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T100733 WP_000323025.1 NZ_CP028166:c821969-821682 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T100733 NZ_CP028166:c821969-821682 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT100733 WP_000534858.1 NZ_CP028166:c822208-821969 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT100733 NZ_CP028166:c822208-821969 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References