Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 754312..755900 Replicon chromosome
Accession NZ_CP028166
Organism Escherichia coli strain CFSAN064036

Toxin (Protein)


Gene name hipA Uniprot ID S1ELM3
Locus tag C6668_RS04040 Protein ID WP_001125439.1
Coordinates 754312..755634 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag C6668_RS04045 Protein ID WP_001301023.1
Coordinates 755634..755900 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6668_RS04005 749408..750322 - 915 WP_000520659.1 fimbrial protein -
C6668_RS04010 750382..750720 - 339 Protein_746 type 1 fimbrial protein -
C6668_RS04015 750805..752033 + 1229 WP_089541817.1 IS3 family transposase -
C6668_RS04020 752045..752221 - 177 Protein_748 type 1 fimbrial protein -
C6668_RS04025 752311..753537 - 1227 Protein_749 fimbria/pilus outer membrane usher protein -
C6668_RS04030 753792..754000 - 209 Protein_750 AraC family transcriptional regulator -
C6668_RS04040 754312..755634 - 1323 WP_001125439.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
C6668_RS04045 755634..755900 - 267 WP_001301023.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
C6668_RS04050 756123..756206 - 84 WP_122988093.1 autotransporter outer membrane beta-barrel domain-containing protein -
C6668_RS04055 756221..756706 + 486 Protein_754 autoinducer 2 ABC transporter permease LsrC -
C6668_RS04060 756706..757698 + 993 WP_001222729.1 autoinducer 2 ABC transporter permease LsrD -
C6668_RS04065 757710..758732 + 1023 WP_000172488.1 autoinducer 2 ABC transporter substrate-binding protein LsrB -
C6668_RS04070 758759..759623 + 865 Protein_757 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase -
C6668_RS04075 759647..759937 + 291 WP_000558527.1 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase -
C6668_RS04080 759994..760752 + 759 WP_001286597.1 trans-aconitate 2-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-401)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49275.82 Da        Isoelectric Point: 8.2482

>T100732 WP_001125439.1 NZ_CP028166:c755634-754312 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

>T100732 NZ_CP028166:c755634-754312 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGTGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT100732 WP_001301023.1 NZ_CP028166:c755900-755634 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp

>AT100732 NZ_CP028166:c755900-755634 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5K98
PDB 3TPV
PDB 3DNV
PDB 3TPD
PDB 3HZI
PDB 3FBR
PDB 4YG7
PDB 3TPE
PDB 2WIU
PDB 3DNT
PDB 3TPB
PDB 3TPT
PDB 3DNU

Antitoxin

Source ID Structure

References