Detailed information of TA system TA100731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 667620..668258
• Replicon: chromosome
• Accession: NZ_CP028166
• Organism: Escherichia coli strain CFSAN064036

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: C6668_RS03650
• Protein ID: WP_000813794.1
• Coordinates: 667620..667796 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C6668_RS03655
• Protein ID: WP_001270286.1
• Coordinates: 667842..668258 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6668_RS03630	663239..664453	-	1215	WP_001326689.1	BenE family transporter YdcO	-
C6668_RS03635	664506..665042	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
C6668_RS03640	665115..667076	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C6668_RS03645	667168..667398	-	231	WP_000494244.1	YncJ family protein	-
C6668_RS03650	667620..667796	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C6668_RS03655	667842..668258	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C6668_RS03660	668337..669743	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
C6668_RS03665	669988..671133	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
C6668_RS03670	671151..672164	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
C6668_RS03675	672165..673106	+	942	WP_107147876.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T100731 WP_000813794.1 NZ_CP028166:667620-667796 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T100731 NZ_CP028166:667620-667796 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT100731 WP_001270286.1 NZ_CP028166:667842-668258 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT100731 NZ_CP028166:667842-668258 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB