Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/- |
| Location | 417261..417895 | Replicon | chromosome |
| Accession | NZ_CP068791 | ||
| Organism | Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain R1 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | Q9RX96 |
| Locus tag | DRO_RS02125 | Protein ID | WP_010887064.1 |
| Coordinates | 417482..417895 (+) | Length | 138 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | - |
| Locus tag | DRO_RS02120 | Protein ID | WP_162177573.1 |
| Coordinates | 417261..417485 (+) | Length | 75 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DRO_RS02085 (DRO_0409) | 412411..413094 | + | 684 | WP_010887056.1 | SDR family oxidoreductase | - |
| DRO_RS02090 (DRO_0410) | 413115..414077 | + | 963 | WP_034349507.1 | NAD(P)/FAD-dependent oxidoreductase | - |
| DRO_RS02095 (DRO_0411) | 414100..414279 | + | 180 | WP_034349506.1 | hypothetical protein | - |
| DRO_RS02100 (DRO_0412) | 414281..414910 | + | 630 | WP_027479568.1 | HAD family hydrolase | - |
| DRO_RS02105 (DRO_0413) | 414958..416478 | - | 1521 | WP_010887060.1 | carboxylesterase/lipase family protein | - |
| DRO_RS02110 (DRO_0414) | 416619..416861 | + | 243 | WP_010887061.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | - |
| DRO_RS02115 (DRO_0415) | 416855..417208 | + | 354 | WP_010887062.1 | endoribonuclease MazF | - |
| DRO_RS02120 (DRO_0416) | 417261..417485 | + | 225 | WP_162177573.1 | hypothetical protein | Antitoxin |
| DRO_RS02125 (DRO_0417) | 417482..417895 | + | 414 | WP_010887064.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| DRO_RS02130 (DRO_0418) | 417892..420360 | + | 2469 | WP_010887065.1 | ATP-dependent helicase HrpB | - |
| DRO_RS02135 (DRO_0419) | 420541..420765 | - | 225 | WP_162177572.1 | hypothetical protein | - |
| DRO_RS02140 (DRO_0420) | 420921..421697 | - | 777 | WP_010887067.1 | trans-aconitate 2-methyltransferase | - |
| DRO_RS02145 (DRO_0421) | 421993..422619 | - | 627 | WP_010887068.1 | single-stranded DNA-binding protein DdrA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 138 a.a. Molecular weight: 14634.68 Da Isoelectric Point: 5.5820
>T10073 WP_010887064.1 NZ_CP068791:417482-417895 [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
MTESTVLDANILSALLRSEANPLSIARQLSALAREGSLVVYAELLAGPEVTPDFLKGFLQEVDVQILPPSGLAVWESAGL
AYGSYAQRRQRSGGGSPRQILSDFVIGAHALYHGAALFTADPQHYRLSFPALTVLTP
MTESTVLDANILSALLRSEANPLSIARQLSALAREGSLVVYAELLAGPEVTPDFLKGFLQEVDVQILPPSGLAVWESAGL
AYGSYAQRRQRSGGGSPRQILSDFVIGAHALYHGAALFTADPQHYRLSFPALTVLTP
Download Length: 414 bp
>T10073 NZ_CP068791:417482-417895 [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
ATGACAGAGAGCACCGTGCTGGACGCCAATATTCTCAGTGCCCTGCTCCGTTCTGAGGCGAACCCTCTGTCCATTGCGCG
TCAGCTTTCGGCGCTGGCCCGTGAGGGTTCACTAGTGGTTTATGCCGAGTTGCTCGCGGGCCCTGAGGTTACGCCCGATT
TCCTCAAGGGATTTCTGCAAGAGGTGGACGTGCAGATTCTTCCGCCGTCTGGCCTTGCGGTCTGGGAAAGCGCGGGCCTC
GCTTATGGGAGCTACGCTCAGCGGCGGCAACGCAGCGGTGGTGGGTCGCCTCGCCAGATTCTGAGTGACTTTGTGATTGG
TGCCCATGCCCTGTATCACGGCGCCGCTCTCTTCACCGCTGATCCTCAGCATTACCGCCTCAGCTTCCCAGCCCTCACAG
TCCTGACCCCATGA
ATGACAGAGAGCACCGTGCTGGACGCCAATATTCTCAGTGCCCTGCTCCGTTCTGAGGCGAACCCTCTGTCCATTGCGCG
TCAGCTTTCGGCGCTGGCCCGTGAGGGTTCACTAGTGGTTTATGCCGAGTTGCTCGCGGGCCCTGAGGTTACGCCCGATT
TCCTCAAGGGATTTCTGCAAGAGGTGGACGTGCAGATTCTTCCGCCGTCTGGCCTTGCGGTCTGGGAAAGCGCGGGCCTC
GCTTATGGGAGCTACGCTCAGCGGCGGCAACGCAGCGGTGGTGGGTCGCCTCGCCAGATTCTGAGTGACTTTGTGATTGG
TGCCCATGCCCTGTATCACGGCGCCGCTCTCTTCACCGCTGATCCTCAGCATTACCGCCTCAGCTTCCCAGCCCTCACAG
TCCTGACCCCATGA
Antitoxin
Download Length: 75 a.a. Molecular weight: 8229.23 Da Isoelectric Point: 4.3481
>AT10073 WP_162177573.1 NZ_CP068791:417261-417485 [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
MTTLEARIENGKVVLTPEQAAELGLREGEVLPVTIAPHEAETEGNPFTRWIGTLPPLPDGEESARFYRRLRDGE
MTTLEARIENGKVVLTPEQAAELGLREGEVLPVTIAPHEAETEGNPFTRWIGTLPPLPDGEESARFYRRLRDGE
Download Length: 225 bp
>AT10073 NZ_CP068791:417261-417485 [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539]
ATGACGACGTTGGAGGCCCGGATTGAGAACGGCAAGGTGGTGCTCACCCCTGAGCAGGCCGCCGAACTGGGCCTGCGCGA
AGGCGAAGTGCTGCCGGTGACGATTGCGCCGCACGAAGCGGAAACGGAAGGCAACCCCTTCACCCGCTGGATTGGGACGC
TCCCCCCACTGCCTGACGGTGAAGAGTCTGCGCGGTTCTACCGCCGCCTGCGAGACGGCGAATGA
ATGACGACGTTGGAGGCCCGGATTGAGAACGGCAAGGTGGTGCTCACCCCTGAGCAGGCCGCCGAACTGGGCCTGCGCGA
AGGCGAAGTGCTGCCGGTGACGATTGCGCCGCACGAAGCGGAAACGGAAGGCAACCCCTTCACCCGCTGGATTGGGACGC
TCCCCCCACTGCCTGACGGTGAAGAGTCTGCGCGGTTCTACCGCCGCCTGCGAGACGGCGAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Immanuel Dhanasingh et al. (2021) Functional and structural characterization of Deinococcus radiodurans R1 MazEF toxin-antitoxin system, Dr0416-Dr0417. Journal of Microbiology (Seoul, Korea) 59(2):186-201. [PubMed:33527318]
(2) Jingli Dai et al. (2021) MazEF Toxin-Antitoxin System-Mediated DNA Damage Stress Response in Deinococcus radiodurans. Frontiers in Genetics 12:632423. [PubMed:33679894]