Detailed information of TA system TA100721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 851975..852504
• Replicon: chromosome
• Accession: NZ_CP028165
• Organism: Staphylococcus aureus strain CFSAN064037

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C9J78_RS04440
• Protein ID: WP_000621175.1
• Coordinates: 851975..852337 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: C9J78_RS04445
• Protein ID: WP_000948331.1
• Coordinates: 852334..852504 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9J78_RS04415	848953..849723	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
C9J78_RS04420	849698..850177	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
C9J78_RS04425	850179..850505	-	327	WP_001052491.1	anti-sigma factor antagonist	-
C9J78_RS04430	850624..851625	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
C9J78_RS04440	851975..852337	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C9J78_RS04445	852334..852504	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
C9J78_RS04450	852589..853737	-	1149	WP_001281143.1	alanine racemase	-
C9J78_RS04455	853803..854162	-	360	WP_000581198.1	holo-ACP synthase	-
C9J78_RS04460	854166..854657	-	492	WP_001205919.1	PH domain-containing protein	-
C9J78_RS04465	854644..856227	-	1584	WP_001294641.1	PH domain-containing protein	-
C9J78_RS04470	856220..856699	-	480	WP_001287083.1	hypothetical protein	-
C9J78_RS04475	856908..857468	-	561	WP_001092415.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T100721 WP_000621175.1 NZ_CP028165:c852337-851975 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T100721 NZ_CP028165:c852337-851975 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGATTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT100721 WP_000948331.1 NZ_CP028165:c852504-852334 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT100721 NZ_CP028165:c852504-852334 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCTATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1