Detailed information of TA system TA100714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1381519..1382295
• Replicon: chromosome
• Accession: NZ_CP028163
• Organism: Staphylococcus aureus strain CFSAN064038

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: C9J77_RS07305
• Protein ID: WP_000031108.1
• Coordinates: 1382143..1382295 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: C9J77_RS07300
• Protein ID: WP_074371037.1
• Coordinates: 1381519..1382118 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9J77_RS07280	1377435..1378892	+	1458	WP_000649908.1	ABC transporter permease subunit	-
C9J77_RS07285	1378885..1379607	+	723	WP_031774878.1	amino acid ABC transporter ATP-binding protein	-
C9J77_RS07290	1379758..1380885	+	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
C9J77_RS07295	1380890..1381360	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
C9J77_RS07300	1381519..1382118	+	600	WP_074371037.1	glucosamine-6-phosphate isomerase	Antitoxin
C9J77_RS07305	1382143..1382295	+	153	WP_000031108.1	hypothetical protein	Toxin
C9J77_RS07315	1382838..1383233	+	396	WP_000901018.1	hypothetical protein	-
C9J77_RS07320	1383429..1384814	+	1386	WP_000116238.1	class II fumarate hydratase	-
C9J77_RS07325	1385269..1386090	-	822	WP_000669380.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T100714 WP_000031108.1 NZ_CP028163:1382143-1382295 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T100714 NZ_CP028163:1382143-1382295 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22355.53 Da
• Isoelectric Point: 5.1445

>AT100714 WP_074371037.1 NZ_CP028163:1381519-1382118 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLILQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT100714 NZ_CP028163:1381519-1382118 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATGTAG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAATATTACAAGTTGTTTCTATCGATGAACAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTTGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
CGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: X5E2E6