Detailed information of TA system TA100710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1186988..1187517
• Replicon: chromosome
• Accession: NZ_CP028163
• Organism: Staphylococcus aureus strain CFSAN064038

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C9J77_RS06070
• Protein ID: WP_000621175.1
• Coordinates: 1187155..1187517 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: C9J77_RS06065
• Protein ID: WP_000948331.1
• Coordinates: 1186988..1187158 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9J77_RS06035	1182024..1182584	+	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-
C9J77_RS06040	1182793..1183272	+	480	WP_001287081.1	hypothetical protein	-
C9J77_RS06045	1183265..1184842	+	1578	WP_001294650.1	PH domain-containing protein	-
C9J77_RS06050	1184835..1185326	+	492	WP_001286980.1	PH domain-containing protein	-
C9J77_RS06055	1185330..1185689	+	360	WP_000581197.1	holo-ACP synthase	-
C9J77_RS06060	1185755..1186903	+	1149	WP_001281139.1	alanine racemase	-
C9J77_RS06065	1186988..1187158	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
C9J77_RS06070	1187155..1187517	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C9J77_RS06080	1187867..1188868	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
C9J77_RS06085	1188987..1189313	+	327	WP_001052491.1	anti-sigma factor antagonist	-
C9J77_RS06090	1189315..1189794	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
C9J77_RS06095	1189769..1190539	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T100710 WP_000621175.1 NZ_CP028163:1187155-1187517 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T100710 NZ_CP028163:1187155-1187517 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT100710 WP_000948331.1 NZ_CP028163:1186988-1187158 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT100710 NZ_CP028163:1186988-1187158 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1