Detailed information of TA system TA100694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2493571..2494613
• Replicon: chromosome
• Accession: NZ_CP028162
• Organism: Pseudomonas aeruginosa strain MRSN12280

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: C8257_RS12150
• Protein ID: WP_003153636.1
• Coordinates: 2494038..2494613 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: C8257_RS12145
• Protein ID: WP_003050245.1
• Coordinates: 2493571..2494041 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C8257_RS12110	2488963..2490381	-	1419	WP_049271526.1	TIGR03752 family integrating conjugative element protein	-
C8257_RS12115	2490371..2491282	-	912	WP_003090194.1	TIGR03749 family integrating conjugative element protein	-
C8257_RS12120	2491279..2491971	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
C8257_RS12125	2491968..2492366	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
C8257_RS12130	2492378..2492737	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
C8257_RS12135	2492754..2492987	-	234	WP_003090170.1	TIGR03758 family integrating conjugative element protein	-
C8257_RS12140	2492984..2493367	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
C8257_RS12145	2493571..2494041	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
C8257_RS12150	2494038..2494613	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
C8257_RS12155	2494631..2495545	+	915	WP_016852809.1	AAA family ATPase	-
C8257_RS12160	2495542..2496012	+	471	WP_003090160.1	hypothetical protein	-
C8257_RS12165	2496009..2496509	+	501	WP_003090159.1	hypothetical protein	-
C8257_RS12170	2496509..2497411	+	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
C8257_RS12175	2497450..2498175	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2421208..2533807	112599

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T100694 WP_003153636.1 NZ_CP028162:2494038-2494613 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T100694 NZ_CP028162:2494038-2494613 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT100694 WP_003050245.1 NZ_CP028162:2493571-2494041 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT100694 NZ_CP028162:2493571-2494041 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68