Detailed information of TA system TA100681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 1285150..1285666
• Replicon: chromosome
• Accession: NZ_CP028141
• Organism: Vibrio parahaemolyticus strain R14

Toxin (Protein)

• Gene name: relB
• Uniprot ID: U2ZZV8
• Locus tag: C9I78_RS06665
• Protein ID: WP_005464459.1
• Coordinates: 1285397..1285666 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: C9I78_RS06660
• Protein ID: WP_025548943.1
• Coordinates: 1285150..1285404 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9I78_RS06625	1281138..1281626	+	489	WP_025549022.1	hypothetical protein	-
C9I78_RS06630	1281763..1282179	+	417	WP_025507116.1	hypothetical protein	-
C9I78_RS26830	1282145..1282294	+	150	WP_162457646.1	DUF3265 domain-containing protein	-
C9I78_RS06640	1282391..1282987	+	597	WP_078532239.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
C9I78_RS06650	1284217..1284372	+	156	Protein_1164	SDR family oxidoreductase	-
C9I78_RS06655	1284660..1284926	+	267	WP_005464625.1	hypothetical protein	-
C9I78_RS06660	1285150..1285404	+	255	WP_025548943.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
C9I78_RS06665	1285397..1285666	+	270	WP_005464459.1	Txe/YoeB family addiction module toxin	Toxin
C9I78_RS06670	1285883..1286104	+	222	Protein_1168	transposase	-
C9I78_RS06675	1286188..1286988	+	801	WP_086482363.1	IS3 family transposase	-
C9I78_RS26725	1287357..1287412	+	56	Protein_1170	DUF3265 domain-containing protein	-
C9I78_RS06685	1287599..1288489	+	891	WP_005485669.1	hypothetical protein	-
C9I78_RS06690	1288570..1288980	+	411	WP_024701711.1	hypothetical protein	-
C9I78_RS06695	1289159..1289592	+	434	Protein_1173	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1223768..1293026	69258
	-	inside	Integron	-	-	1224292..1289125	64833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (10-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10614.96 Da
• Isoelectric Point: 7.9816

>T100681 WP_005464459.1 NZ_CP028141:1285397-1285666 [Vibrio parahaemolyticus]
MSSSQRLLSWTDDAWDDYLYWQTQDKKTLKRINKLINDVKRSPFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDQAI
TIISCRYHY

Nucleotide

• Length: 270 bp

>T100681 NZ_CP028141:1285397-1285666 [Vibrio parahaemolyticus]
ATGAGTAGTAGTCAACGTTTATTATCGTGGACTGATGATGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAATAAACTCATCAATGATGTTAAGCGCTCTCCATTTGAGGGCATTGGTAAACCAGAGCCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTATTGATGATACTAATAGGCTTGTTTACGCAGTCGATGATCAAGCGATA
ACGATAATTTCATGTCGTTACCACTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9511.68 Da
• Isoelectric Point: 5.0623

>AT100681 WP_025548943.1 NZ_CP028141:1285150-1285404 [Vibrio parahaemolyticus]
MRIVSFTEARNSLKAVLDGVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPQNVEHLNRSIAQYRAGKTTARE
LIDE

Nucleotide

• Length: 255 bp

>AT100681 NZ_CP028141:1285150-1285404 [Vibrio parahaemolyticus]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATAGTCTCAAAGCTGTTTTAGACGGTGTAGTTAATGATGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAGGATGCAGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACTGTTCATT
TACTACGCTCTCCTCAAAACGTTGAACACCTAAACCGTTCGATAGCACAGTACCGCGCTGGTAAAACAACAGCACGAGAG
TTAATTGATGAGTAG

Structures

Toxin

• AlphaFold DB: U2ZZV8