Detailed information of TA system TA010068

Experimentally validated

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/-
• Location: 925787..926440
• Replicon: chromosome
• Accession: NZ_CP018664
• Organism: Acinetobacter baumannii strain ATCC 17978

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A1E3M967
• Locus tag: AUO97_RS04255
• Protein ID: WP_009518132.1
• Coordinates: 925787..926176 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: V5V966
• Locus tag: AUO97_RS04260
• Protein ID: WP_001288210.1
• Coordinates: 926183..926440 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AUO97_RS04240 (AUO97_04240)	920904..923099	-	2196	WP_001158904.1	TRAP transporter large permease subunit	-
AUO97_RS04245 (AUO97_04245)	923287..923853	-	567	WP_000651535.1	rhombosortase	-
AUO97_RS04250 (AUO97_04250)	923932..925017	-	1086	WP_000049106.1	hypothetical protein	-
AUO97_RS04255 (AUO97_04255)	925787..926176	-	390	WP_009518132.1	hypothetical protein	Toxin
AUO97_RS04260 (AUO97_04260)	926183..926440	-	258	WP_001288210.1	succinate dehydrogenase assembly factor 2	Antitoxin
AUO97_RS04265 (AUO97_04265)	926628..927800	+	1173	WP_001190550.1	acyl-CoA dehydrogenase family protein	-
AUO97_RS04270 (AUO97_04270)	927850..929340	-	1491	WP_000415133.1	NAD(P)/FAD-dependent oxidoreductase	-
AUO97_RS04275 (AUO97_04275)	929522..929899	-	378	WP_001216380.1	50S ribosomal protein L17	-
AUO97_RS04280 (AUO97_04280)	929918..930925	-	1008	WP_000198631.1	DNA-directed RNA polymerase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Flavinator of succinate dehydrogenase (10-82)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 15720.03 Da
• Isoelectric Point: 10.4623

>T10068 WP_009518132.1 NZ_CP018664:c926176-925787 [Acinetobacter baumannii]
MINHLNFKLKYSRFSIIFQFFIGLSLAVLFYQLMPLLWWLVVISLLFINFIFFLKRPQLAQIAYLDKKLWSLAYHSQNTI
SRVKILKIIDYQIFIVIYFEGHKKLTSIIWFDQMSLTEWKKLKTLEKLY

Nucleotide

• Length: 390 bp

>T10068 NZ_CP018664:c926176-925787 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAACTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCATTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAAAACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAACAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 10318.77 Da
• Isoelectric Point: 4.6150

>AT10068 WP_001288210.1 NZ_CP018664:c926440-926183 [Acinetobacter baumannii]
MSEEMTLEERKVIYRARRGLKEIDVYFDPYVKNYYLKADPAEKALFAELVEQEDPDLLDWFMEVSEPPRTELRDFIYKLK
HYVHG

Nucleotide

• Length: 258 bp

>AT10068 NZ_CP018664:c926440-926183 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT10045	Shewanella oneidensis MR-1	38.571	85.366	0.329

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: A0A1E3M967

Antitoxin

• AlphaFold DB: A0A7U4BQM7

References

(1) Shahira A ElBanna et al. (2021) Genomics-guided identification of a conserved CptBA-like toxin-antitoxin system in Acinetobacter baumannii. Journal of Advanced Research 30:159-170. [PubMed:34026293]


 experimental literature