Detailed information of TA system TA100661

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5670307..5671139
• Replicon: chromosome
• Accession: NZ_CP028126
• Organism: Escherichia coli O26 str. RM10386

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: I3U_RS32055
• Protein ID: WP_000854753.1
• Coordinates: 5670765..5671139 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: I3U_RS32050
• Protein ID: WP_001315620.1
• Coordinates: 5670307..5670675 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U_RS32015	5667137..5667592	+	456	WP_000581504.1	hypothetical protein	-
I3U_RS32020	5667671..5667904	+	234	WP_001119729.1	DUF905 family protein	-
I3U_RS32030	5668004..5668822	+	819	WP_001234622.1	DUF945 domain-containing protein	-
I3U_RS32035	5668877..5669362	+	486	WP_000849582.1	antirestriction protein	-
I3U_RS32040	5669378..5669854	+	477	WP_001186725.1	RadC family protein	-
I3U_RS32045	5669923..5670144	+	222	WP_000692323.1	DUF987 domain-containing protein	-
I3U_RS32050	5670307..5670675	+	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I3U_RS32055	5670765..5671139	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
I3U_RS32060	5671136..5671624	+	489	WP_000777547.1	hypothetical protein	-
I3U_RS32065	5671636..5671833	+	198	WP_000839281.1	DUF957 domain-containing protein	-
I3U_RS33490	5671918..5672070	+	153	WP_001280445.1	hypothetical protein	-
I3U_RS32090	5673115..5673765	+	651	WP_001037966.1	HNH endonuclease	-
I3U_RS32095	5673773..5674753	-	981	WP_000991415.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
I3U_RS32100	5674818..5675924	-	1107	WP_001341302.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	5661724..5679792	18068

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T100661 WP_000854753.1 NZ_CP028126:5670765-5671139 [Escherichia coli O26 str. RM10386]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T100661 NZ_CP028126:5670765-5671139 [Escherichia coli O26 str. RM10386]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT100661 WP_001315620.1 NZ_CP028126:5670307-5670675 [Escherichia coli O26 str. RM10386]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT100661 NZ_CP028126:5670307-5670675 [Escherichia coli O26 str. RM10386]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5