Detailed information of TA system TA100653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3528998..3529623
• Replicon: chromosome
• Accession: NZ_CP028126
• Organism: Escherichia coli O26 str. RM10386

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I3U_RS20910
• Protein ID: WP_000911330.1
• Coordinates: 3528998..3529396 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: I3U_RS20915
• Protein ID: WP_000450524.1
• Coordinates: 3529396..3529623 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U_RS20890	3524876..3525076	+	201	WP_000383836.1	YpfN family protein	-
I3U_RS20895	3525186..3525884	-	699	WP_000679812.1	esterase	-
I3U_RS20900	3525958..3527973	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
I3U_RS20905	3527988..3528851	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
I3U_RS20910	3528998..3529396	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
I3U_RS20915	3529396..3529623	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
I3U_RS20920	3529777..3530490	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
I3U_RS20925	3530703..3531737	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
I3U_RS20930	3531754..3532632	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
I3U_RS20935	3532778..3533350	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
I3U_RS20940	3533350..3533820	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T100653 WP_000911330.1 NZ_CP028126:c3529396-3528998 [Escherichia coli O26 str. RM10386]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T100653 NZ_CP028126:c3529396-3528998 [Escherichia coli O26 str. RM10386]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT100653 WP_000450524.1 NZ_CP028126:c3529623-3529396 [Escherichia coli O26 str. RM10386]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT100653 NZ_CP028126:c3529623-3529396 [Escherichia coli O26 str. RM10386]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33