Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2593316..2593787 Replicon chromosome
Accession NZ_CP028126
Organism Escherichia coli O26 str. RM10386

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A1Y2Y8G6
Locus tag I3U_RS15810 Protein ID WP_001448501.1
Coordinates 2593509..2593787 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag I3U_RS15805 Protein ID WP_001302048.1
Coordinates 2593316..2593507 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3U_RS15755 2588394..2588612 - 219 WP_000683609.1 DUF4014 family protein -
I3U_RS15760 2588740..2589051 - 312 WP_001289673.1 hypothetical protein -
I3U_RS15765 2589044..2589271 - 228 WP_000699809.1 hypothetical protein -
I3U_RS15770 2589268..2589549 - 282 WP_000603384.1 DNA-binding protein -
I3U_RS15775 2589582..2590343 - 762 WP_000450872.1 DUF1627 domain-containing protein -
I3U_RS15780 2590369..2591110 - 742 Protein_2683 ATP-binding protein -
I3U_RS15785 2591117..2592079 - 963 WP_001444941.1 helix-turn-helix domain-containing protein -
I3U_RS15790 2592102..2592527 - 426 WP_000693943.1 Rha family transcriptional regulator -
I3U_RS15795 2592524..2592826 - 303 WP_000172738.1 transcriptional regulator -
I3U_RS15800 2592924..2593295 + 372 WP_001169687.1 hypothetical protein -
I3U_RS15805 2593316..2593507 + 192 WP_001302048.1 hypothetical protein Antitoxin
I3U_RS15810 2593509..2593787 + 279 WP_001448501.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I3U_RS15815 2594074..2594226 + 153 WP_001427316.1 DUF1391 family protein -
I3U_RS15820 2594647..2594868 + 222 WP_000560218.1 killing protein KilR -
I3U_RS15825 2594862..2595038 + 177 WP_000245534.1 hypothetical protein -
I3U_RS15830 2595112..2595387 + 276 WP_001307773.1 hypothetical protein -
I3U_RS15835 2595486..2598137 + 2652 WP_000105101.1 exodeoxyribonuclease VIII -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 2535694..2606667 70973


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10691.27 Da        Isoelectric Point: 5.5647

>T100642 WP_001448501.1 NZ_CP028126:2593509-2593787 [Escherichia coli O26 str. RM10386]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPSLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T100642 NZ_CP028126:2593509-2593787 [Escherichia coli O26 str. RM10386]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTAGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT100642 WP_001302048.1 NZ_CP028126:2593316-2593507 [Escherichia coli O26 str. RM10386]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT100642 NZ_CP028126:2593316-2593507 [Escherichia coli O26 str. RM10386]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y2Y8G6

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References