Detailed information of TA system TA100639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2037529..2038167
• Replicon: chromosome
• Accession: NZ_CP028126
• Organism: Escherichia coli O26 str. RM10386

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: I3U_RS12635
• Protein ID: WP_000813794.1
• Coordinates: 2037529..2037705 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I3U_RS12640
• Protein ID: WP_001270286.1
• Coordinates: 2037751..2038167 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U_RS12615	2033148..2034362	-	1215	WP_001307190.1	BenE family transporter YdcO	-
I3U_RS12620	2034415..2034951	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
I3U_RS12625	2035024..2036985	+	1962	WP_001341357.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I3U_RS12630	2037077..2037307	-	231	WP_000494244.1	YncJ family protein	-
I3U_RS12635	2037529..2037705	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I3U_RS12640	2037751..2038167	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I3U_RS12645	2038246..2039651	+	1406	Protein_2105	PLP-dependent aminotransferase family protein	-
I3U_RS12650	2039896..2041041	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
I3U_RS12655	2041059..2042072	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
I3U_RS12660	2042073..2043014	+	942	WP_001251332.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2031960..2033108	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T100639 WP_000813794.1 NZ_CP028126:2037529-2037705 [Escherichia coli O26 str. RM10386]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T100639 NZ_CP028126:2037529-2037705 [Escherichia coli O26 str. RM10386]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT100639 WP_001270286.1 NZ_CP028126:2037751-2038167 [Escherichia coli O26 str. RM10386]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT100639 NZ_CP028126:2037751-2038167 [Escherichia coli O26 str. RM10386]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB