Detailed information of TA system TA100627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 35127..35728
• Replicon: plasmid pRM10386-1
• Accession: NZ_CP028125
• Organism: Escherichia coli O26 str. RM10386

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: I3U_RS00800
• Protein ID: WP_001216045.1
• Coordinates: 35348..35728 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: I3U_RS00795
• Protein ID: WP_001190712.1
• Coordinates: 35127..35348 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U_RS00740	30248..30436	+	189	WP_000797279.1	hypothetical protein	-
I3U_RS00745	30438..30647	+	210	WP_000951710.1	hypothetical protein	-
I3U_RS00750	30644..31186	+	543	WP_021351680.1	DUF551 domain-containing protein	-
I3U_RS00755	31269..31502	+	234	WP_000516537.1	hypothetical protein	-
I3U_RS00760	31681..31974	+	294	WP_000269003.1	hypothetical protein	-
I3U_RS00765	31981..32355	+	375	WP_000988658.1	hypothetical protein	-
I3U_RS00770	32337..33269	+	933	WP_000057449.1	hypothetical protein	-
I3U_RS00775	33266..33628	+	363	WP_001261544.1	hypothetical protein	-
I3U_RS33225	33703..33840	-	138	WP_000123562.1	hypothetical protein	-
I3U_RS00785	34290..34541	+	252	WP_001377386.1	DNA polymerase III subunit theta	-
I3U_RS00790	34665..35054	+	390	WP_000506726.1	DNA repair protein	-
I3U_RS00795	35127..35348	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I3U_RS00800	35348..35728	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
I3U_RS00805	35733..35912	+	180	WP_000113019.1	hypothetical protein	-
I3U_RS00810	35940..36983	+	1044	WP_000648825.1	DUF968 domain-containing protein	-
I3U_RS00815	37072..37524	+	453	WP_001369802.1	hypothetical protein	-
I3U_RS00820	37611..38804	+	1194	WP_109041926.1	terminase	-
I3U_RS00825	38804..40288	+	1485	WP_000124155.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..98899	98899

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T100627 WP_001216045.1 NZ_CP028125:35348-35728 [Escherichia coli O26 str. RM10386]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T100627 NZ_CP028125:35348-35728 [Escherichia coli O26 str. RM10386]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT100627 WP_001190712.1 NZ_CP028125:35127-35348 [Escherichia coli O26 str. RM10386]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT100627 NZ_CP028125:35127-35348 [Escherichia coli O26 str. RM10386]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6