Detailed information of TA system TA100626

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 9821..10464
• Replicon: plasmid pRM10386-2
• Accession: NZ_CP028124
• Organism: Escherichia coli O26 str. RM10386

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: I3U_RS00065
• Protein ID: WP_001044768.1
• Coordinates: 10048..10464 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: I3U_RS00060
• Protein ID: WP_001261287.1
• Coordinates: 9821..10051 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3U_RS00020	5687..7126	+	1440	WP_001213545.1	enterohemolysin T1SS ABC transporter subunit EhxD	-
I3U_RS00025	7193..7336	+	144	Protein_4	transcriptional regulator	-
I3U_RS00030	7340..7531	+	192	Protein_5	lipid II-degrading bacteriocin	-
I3U_RS00035	7539..7892	-	354	WP_000864810.1	colicin M immunity protein	-
I3U_RS00040	8065..8847	-	783	WP_001164205.1	site-specific integrase	-
I3U_RS00045	8849..9262	-	414	WP_000465041.1	hypothetical protein	-
I3U_RS00060	9821..10051	+	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I3U_RS00065	10048..10464	+	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
I3U_RS00070	10626..11171	-	546	WP_001165114.1	hypothetical protein	-
I3U_RS00075	11239..12395	+	1157	WP_085948186.1	IS3-like element IS600 family transposase	-
I3U_RS33175	12436..12585	-	150	WP_000955366.1	hypothetical protein	-
I3U_RS00080	13200..14060	-	861	WP_109041911.1	alpha/beta hydrolase	-
I3U_RS00085	14188..14574	+	387	WP_001066949.1	tyrosine-type recombinase/integrase	-
I3U_RS00090	14628..15302	+	675	WP_001341423.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD / toxB / toxB / katA / espP	1..83012	83012

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T100626 WP_001044768.1 NZ_CP028124:10048-10464 [Escherichia coli O26 str. RM10386]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T100626 NZ_CP028124:10048-10464 [Escherichia coli O26 str. RM10386]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT100626 WP_001261287.1 NZ_CP028124:9821-10051 [Escherichia coli O26 str. RM10386]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT100626 NZ_CP028124:9821-10051 [Escherichia coli O26 str. RM10386]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4