Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4711136..4711633 Replicon chromosome
Accession NZ_CP028122
Organism Escherichia coli O43 str. RM10042

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I3W_RS25630 Protein ID WP_001173343.1
Coordinates 4711460..4711633 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I3W_RS25625 Protein ID WP_000398619.1
Coordinates 4711136..4711432 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3W_RS25605 4707867..4708586 - 720 WP_000611288.1 two-component system response regulator DcuR -
I3W_RS25610 4708583..4710214 - 1632 WP_001216487.1 sensor histidine kinase -
I3W_RS25615 4710395..4710625 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
I3W_RS25620 4710637..4710909 + 273 WP_000405649.1 N-acetyltransferase -
I3W_RS25625 4711136..4711432 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I3W_RS25630 4711460..4711633 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I3W_RS25635 4711752..4713269 - 1518 WP_001295087.1 lysine--tRNA ligase -
I3W_RS25640 4713506..4714963 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T100623 WP_001173343.1 NZ_CP028122:4711460-4711633 [Escherichia coli O43 str. RM10042]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T100623 NZ_CP028122:4711460-4711633 [Escherichia coli O43 str. RM10042]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT100623 WP_000398619.1 NZ_CP028122:4711136-4711432 [Escherichia coli O43 str. RM10042]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT100623 NZ_CP028122:4711136-4711432 [Escherichia coli O43 str. RM10042]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References