Detailed information of TA system TA100620

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3500813..3501645
• Replicon: chromosome
• Accession: NZ_CP028122
• Organism: Escherichia coli O43 str. RM10042

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2A2C6E0
• Locus tag: I3W_RS19460
• Protein ID: WP_000854716.1
• Coordinates: 3500813..3501187 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I3W_RS19465
• Protein ID: WP_108942479.1
• Coordinates: 3501277..3501645 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3W_RS19430	3496460..3498301	+	1842	WP_108942477.1	RNA polymerase sigma factor RpoD	-
I3W_RS19435	3498380..3498886	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
I3W_RS19445	3499186..3500031	-	846	WP_001280505.1	DUF4942 domain-containing protein	-
I3W_RS19450	3500116..3500313	-	198	WP_000839249.1	DUF957 domain-containing protein	-
I3W_RS19455	3500325..3500816	-	492	WP_000976843.1	hypothetical protein	-
I3W_RS19460	3500813..3501187	-	375	WP_000854716.1	TA system toxin CbtA family protein	Toxin
I3W_RS19465	3501277..3501645	-	369	WP_108942479.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
I3W_RS19470	3501703..3501942	-	240	WP_000692304.1	DUF987 domain-containing protein	-
I3W_RS19475	3502005..3502481	-	477	WP_162617009.1	RadC family protein	-
I3W_RS19480	3502497..3502982	-	486	WP_000213718.1	antirestriction protein	-
I3W_RS19485	3503074..3503892	-	819	WP_033801547.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13986.02 Da
• Isoelectric Point: 7.2426

>T100620 WP_000854716.1 NZ_CP028122:c3501187-3500813 [Escherichia coli O43 str. RM10042]
MKTLPDTHVREASRCPSPVIIWQILLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYLEAK

Nucleotide

• Length: 375 bp

>T100620 NZ_CP028122:c3501187-3500813 [Escherichia coli O43 str. RM10042]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCATCATCTGGCAGATTCTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAATTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATCCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCTGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13479.36 Da
• Isoelectric Point: 6.3132

>AT100620 WP_108942479.1 NZ_CP028122:c3501645-3501277 [Escherichia coli O43 str. RM10042]
VSDTLPGTTLPDDNHDRPWWGLPCTVTSCFGARLVQVGNRLHYLADRAGIRGLFSDADAYHLDLAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT100620 NZ_CP028122:c3501645-3501277 [Escherichia coli O43 str. RM10042]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GTCCTGTTTCGGCGCCCGTCTGGTGCAGGTGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCTGGCCTTTCCGCTGTTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCTAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACCCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2A2C6E0