Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3299030..3299684 Replicon chromosome
Accession NZ_CP028122
Organism Escherichia coli O43 str. RM10042

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag I3W_RS18370 Protein ID WP_000244772.1
Coordinates 3299030..3299437 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I3W_RS18375 Protein ID WP_000354046.1
Coordinates 3299418..3299684 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3W_RS18350 3294987..3296720 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
I3W_RS18355 3296726..3297436 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I3W_RS18360 3297461..3298357 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I3W_RS18365 3298469..3298990 + 522 WP_001055874.1 flavodoxin FldB -
I3W_RS18370 3299030..3299437 - 408 WP_000244772.1 protein YgfX Toxin
I3W_RS18375 3299418..3299684 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I3W_RS18380 3299927..3300907 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
I3W_RS18385 3301103..3301762 - 660 WP_000250274.1 hemolysin III family protein -
I3W_RS18390 3301926..3302237 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
I3W_RS18395 3302282..3303715 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
I3W_RS18400 3303772..3304515 - 744 WP_021554523.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T100619 WP_000244772.1 NZ_CP028122:c3299437-3299030 [Escherichia coli O43 str. RM10042]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T100619 NZ_CP028122:c3299437-3299030 [Escherichia coli O43 str. RM10042]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT100619 WP_000354046.1 NZ_CP028122:c3299684-3299418 [Escherichia coli O43 str. RM10042]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT100619 NZ_CP028122:c3299684-3299418 [Escherichia coli O43 str. RM10042]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References