Detailed information of TA system TA100605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1615602..1616240
• Replicon: chromosome
• Accession: NZ_CP028122
• Organism: Escherichia coli O43 str. RM10042

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: I3W_RS09600
• Protein ID: WP_000813794.1
• Coordinates: 1615602..1615778 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I3W_RS09605
• Protein ID: WP_001270285.1
• Coordinates: 1615824..1616240 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3W_RS09580	1611221..1612435	-	1215	WP_001307190.1	BenE family transporter YdcO	-
I3W_RS09585	1612488..1613024	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
I3W_RS09590	1613097..1615058	+	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I3W_RS09595	1615150..1615380	-	231	WP_000494244.1	YncJ family protein	-
I3W_RS09600	1615602..1615778	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I3W_RS09605	1615824..1616240	+	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I3W_RS09610	1616319..1617725	+	1407	WP_096968129.1	PLP-dependent aminotransferase family protein	-
I3W_RS09615	1617970..1619115	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
I3W_RS09620	1619133..1620146	+	1014	WP_000220412.1	ABC transporter ATP-binding protein	-
I3W_RS09625	1620147..1621088	+	942	WP_001251319.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1609916..1611181	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T100605 WP_000813794.1 NZ_CP028122:1615602-1615778 [Escherichia coli O43 str. RM10042]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T100605 NZ_CP028122:1615602-1615778 [Escherichia coli O43 str. RM10042]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT100605 WP_001270285.1 NZ_CP028122:1615824-1616240 [Escherichia coli O43 str. RM10042]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT100605 NZ_CP028122:1615824-1616240 [Escherichia coli O43 str. RM10042]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB