Detailed information of TA system TA100597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 455858..456695
• Replicon: chromosome
• Accession: NZ_CP028122
• Organism: Escherichia coli O43 str. RM10042

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: I3W_RS03465
• Protein ID: WP_000227784.1
• Coordinates: 455858..456400 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: I3W_RS03470
• Protein ID: WP_001297137.1
• Coordinates: 456384..456695 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3W_RS03445	451397..452308	-	912	WP_099145005.1	2-dehydropantoate 2-reductase	-
I3W_RS03450	452476..452967	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
I3W_RS03455	453095..454459	-	1365	WP_001000978.1	MFS transporter	-
I3W_RS03460	454867..455802	+	936	WP_001297127.1	sel1 repeat family protein	-
I3W_RS03465	455858..456400	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
I3W_RS03470	456384..456695	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
I3W_RS03475	456880..457770	-	891	WP_000971336.1	heme o synthase	-
I3W_RS03480	457782..458111	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
I3W_RS03485	458111..458725	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
I3W_RS03490	458715..460706	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
I3W_RS03495	460728..461675	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T100597 WP_000227784.1 NZ_CP028122:c456400-455858 [Escherichia coli O43 str. RM10042]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T100597 NZ_CP028122:c456400-455858 [Escherichia coli O43 str. RM10042]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT100597 WP_001297137.1 NZ_CP028122:c456695-456384 [Escherichia coli O43 str. RM10042]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT100597 NZ_CP028122:c456695-456384 [Escherichia coli O43 str. RM10042]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3