Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 80482..81007 Replicon plasmid pRM10042-2
Accession NZ_CP028121
Organism Escherichia coli O43 str. RM10042

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag I3W_RS01065 Protein ID WP_001159871.1
Coordinates 80482..80787 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag I3W_RS01070 Protein ID WP_000813639.1
Coordinates 80789..81007 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3W_RS01030 75749..77019 + 1271 Protein_74 translesion error-prone DNA polymerase V subunit UmuC -
I3W_RS01040 77024..77416 - 393 WP_000340836.1 plasmid partitioning/stability family protein -
I3W_RS01045 77421..78392 - 972 WP_001103695.1 hypothetical protein -
I3W_RS01050 78621..79265 + 645 WP_000633912.1 AAA family ATPase -
I3W_RS01055 79259..79534 + 276 WP_000239527.1 hypothetical protein -
I3W_RS01060 79672..80481 - 810 WP_000016958.1 site-specific integrase -
I3W_RS01065 80482..80787 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
I3W_RS01070 80789..81007 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I3W_RS01075 81566..81826 + 261 WP_000434493.1 hypothetical protein -
I3W_RS01080 81823..82413 + 591 WP_000194552.1 hypothetical protein -
I3W_RS01085 82431..82778 - 348 WP_000142428.1 hypothetical protein -
I3W_RS01095 83259..83630 - 372 WP_001373114.1 colicin -
I3W_RS01100 83690..84421 - 732 WP_000850415.1 complement resistance protein TraT -
I3W_RS01110 84693..84803 - 111 Protein_87 IS481 family transposase -
I3W_RS27860 84807..85028 - 222 Protein_88 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - espP / espP / espP / hlyD / hlyB / hlyB / hlyA / hlyC 1..109466 109466


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T100595 WP_001159871.1 NZ_CP028121:c80787-80482 [Escherichia coli O43 str. RM10042]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T100595 NZ_CP028121:c80787-80482 [Escherichia coli O43 str. RM10042]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCTGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT100595 WP_000813639.1 NZ_CP028121:c81007-80789 [Escherichia coli O43 str. RM10042]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT100595 NZ_CP028121:c81007-80789 [Escherichia coli O43 str. RM10042]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References