Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 74546..75093 Replicon plasmid pRM10042-1
Accession NZ_CP028120
Organism Escherichia coli O43 str. RM10042

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag I3W_RS00495 Protein ID WP_000969996.1
Coordinates 74546..74827 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID F4V9S6
Locus tag I3W_RS00500 Protein ID WP_000079931.1
Coordinates 74824..75093 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3W_RS00460 69816..70229 - 414 WP_023210874.1 hypothetical protein -
I3W_RS00465 70797..71057 + 261 WP_019842588.1 hypothetical protein -
I3W_RS00470 71054..71644 + 591 WP_023166813.1 hypothetical protein -
I3W_RS00480 72247..72840 + 594 WP_080193736.1 proQ/FINO family protein -
I3W_RS00485 73122..73709 - 588 WP_032274974.1 serine acetyltransferase -
I3W_RS00490 74009..74350 + 342 WP_000578011.1 hypothetical protein -
I3W_RS00495 74546..74827 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I3W_RS00500 74824..75093 - 270 WP_000079931.1 hypothetical protein Antitoxin
I3W_RS00515 76002..77033 - 1032 WP_108942190.1 replication initiation protein -
I3W_RS00530 77734..78375 + 642 WP_000335846.1 transcription termination factor NusG -
I3W_RS00535 78516..79181 + 666 WP_100033550.1 traC -
I3W_RS00545 79448..80068 + 621 WP_000176499.1 DUF1449 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..86617 86617


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T100590 WP_000969996.1 NZ_CP028120:c74827-74546 [Escherichia coli O43 str. RM10042]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T100590 NZ_CP028120:c74827-74546 [Escherichia coli O43 str. RM10042]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCAGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTATTGCATACCGCACAGAAGTGGCCATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10194.55 Da        Isoelectric Point: 8.8735

>AT100590 WP_000079931.1 NZ_CP028120:c75093-74824 [Escherichia coli O43 str. RM10042]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT100590 NZ_CP028120:c75093-74824 [Escherichia coli O43 str. RM10042]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CTGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DLR5

References