Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3449295..3449949 Replicon chromosome
Accession NZ_CP028117
Organism Escherichia coli O111 str. RM9322

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag I3O_RS18435 Protein ID WP_000244777.1
Coordinates 3449295..3449702 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I3O_RS18440 Protein ID WP_000354046.1
Coordinates 3449683..3449949 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3O_RS18415 3445252..3446985 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
I3O_RS18420 3446991..3447701 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I3O_RS18425 3447726..3448622 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I3O_RS18430 3448734..3449255 + 522 WP_001055874.1 flavodoxin FldB -
I3O_RS18435 3449295..3449702 - 408 WP_000244777.1 toxin CptA Toxin
I3O_RS18440 3449683..3449949 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I3O_RS18445 3450192..3451172 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
I3O_RS18450 3451368..3452027 - 660 WP_000250274.1 hemolysin III family protein -
I3O_RS18455 3452191..3452502 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
I3O_RS18460 3452547..3453980 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
I3O_RS18465 3454037..3454780 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T100580 WP_000244777.1 NZ_CP028117:c3449702-3449295 [Escherichia coli O111 str. RM9322]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T100580 NZ_CP028117:c3449702-3449295 [Escherichia coli O111 str. RM9322]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT100580 WP_000354046.1 NZ_CP028117:c3449949-3449683 [Escherichia coli O111 str. RM9322]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT100580 NZ_CP028117:c3449949-3449683 [Escherichia coli O111 str. RM9322]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References