Detailed information of TA system TA100577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2976455..2977080
• Replicon: chromosome
• Accession: NZ_CP028117
• Organism: Escherichia coli O111 str. RM9322

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I3O_RS15995
• Protein ID: WP_000911330.1
• Coordinates: 2976455..2976853 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: I3O_RS16000
• Protein ID: WP_000450524.1
• Coordinates: 2976853..2977080 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3O_RS15975	2972333..2972533	+	201	WP_000383836.1	YpfN family protein	-
I3O_RS15980	2972643..2973341	-	699	WP_000679812.1	esterase	-
I3O_RS15985	2973415..2975430	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
I3O_RS15990	2975445..2976308	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
I3O_RS15995	2976455..2976853	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
I3O_RS16000	2976853..2977080	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
I3O_RS16005	2977234..2977947	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
I3O_RS16010	2978160..2979194	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
I3O_RS16015	2979211..2980089	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
I3O_RS16020	2980235..2980819	+	585	WP_000176194.1	glycine cleavage system transcriptional repressor	-
I3O_RS16025	2980819..2981289	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T100577 WP_000911330.1 NZ_CP028117:c2976853-2976455 [Escherichia coli O111 str. RM9322]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T100577 NZ_CP028117:c2976853-2976455 [Escherichia coli O111 str. RM9322]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT100577 WP_000450524.1 NZ_CP028117:c2977080-2976853 [Escherichia coli O111 str. RM9322]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT100577 NZ_CP028117:c2977080-2976853 [Escherichia coli O111 str. RM9322]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33