Detailed information of TA system TA100567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1851317..1851955
• Replicon: chromosome
• Accession: NZ_CP028117
• Organism: Escherichia coli O111 str. RM9322

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: I3O_RS09965
• Protein ID: WP_000813794.1
• Coordinates: 1851779..1851955 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I3O_RS09960
• Protein ID: WP_001270286.1
• Coordinates: 1851317..1851733 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I3O_RS09940	1846469..1847410	-	942	WP_001251315.1	ABC transporter permease	-
I3O_RS09945	1847411..1848424	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
I3O_RS09950	1848442..1849587	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
I3O_RS09955	1849832..1851238	-	1407	WP_000760637.1	PLP-dependent aminotransferase family protein	-
I3O_RS09960	1851317..1851733	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I3O_RS09965	1851779..1851955	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I3O_RS09970	1852177..1852407	+	231	WP_000494244.1	YncJ family protein	-
I3O_RS09975	1852499..1854460	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I3O_RS09980	1854533..1855069	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
I3O_RS09985	1855122..1856330	+	1209	WP_071532666.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1856370..1857518	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T100567 WP_000813794.1 NZ_CP028117:c1851955-1851779 [Escherichia coli O111 str. RM9322]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T100567 NZ_CP028117:c1851955-1851779 [Escherichia coli O111 str. RM9322]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT100567 WP_001270286.1 NZ_CP028117:c1851733-1851317 [Escherichia coli O111 str. RM9322]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT100567 NZ_CP028117:c1851733-1851317 [Escherichia coli O111 str. RM9322]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB