Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1350962..1351433 Replicon chromosome
Accession NZ_CP028117
Organism Escherichia coli O111 str. RM9322

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag I3O_RS07100 Protein ID WP_001303511.1
Coordinates 1350962..1351240 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag I3O_RS07105 Protein ID WP_001302048.1
Coordinates 1351242..1351433 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3O_RS07060 1346269..1346538 - 270 WP_000003742.1 excisionase -
I3O_RS07065 1346600..1349065 - 2466 WP_000048442.1 exonuclease -
I3O_RS07070 1349158..1349349 - 192 WP_001090200.1 DUF1482 family protein -
I3O_RS07075 1349346..1349534 - 189 WP_000449192.1 cell division inhibition protein DicB -
I3O_RS07085 1350018..1350236 - 219 WP_001171930.1 hypothetical protein -
I3O_RS07090 1350277..1350666 - 390 WP_001447517.1 hypothetical protein -
I3O_RS07100 1350962..1351240 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I3O_RS07105 1351242..1351433 - 192 WP_001302048.1 hypothetical protein Antitoxin
I3O_RS07110 1351454..1351825 - 372 WP_001169687.1 hypothetical protein -
I3O_RS07115 1351923..1352225 + 303 WP_001444689.1 transcriptional regulator -
I3O_RS07120 1352222..1352647 + 426 WP_000693943.1 Rha family transcriptional regulator -
I3O_RS07125 1352670..1353632 + 963 WP_000095671.1 helix-turn-helix domain-containing protein -
I3O_RS07130 1353673..1354089 + 417 WP_023981635.1 DUF977 family protein -
I3O_RS07145 1355652..1355963 + 312 WP_001002672.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espX7/nleL / espN / espK 1345182..1404965 59783


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T100561 WP_001303511.1 NZ_CP028117:c1351240-1350962 [Escherichia coli O111 str. RM9322]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T100561 NZ_CP028117:c1351240-1350962 [Escherichia coli O111 str. RM9322]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT100561 WP_001302048.1 NZ_CP028117:c1351433-1351242 [Escherichia coli O111 str. RM9322]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT100561 NZ_CP028117:c1351433-1351242 [Escherichia coli O111 str. RM9322]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References