Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1256409..1257204 Replicon chromosome
Accession NZ_CP028117
Organism Escherichia coli O111 str. RM9322

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag I3O_RS06545 Protein ID WP_000854914.1
Coordinates 1256830..1257204 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag I3O_RS06540 Protein ID WP_001280954.1
Coordinates 1256409..1256783 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3O_RS06505 1253416..1253736 + 321 WP_000149392.1 phospholipase -
I3O_RS06520 1254076..1254894 + 819 WP_001234682.1 DUF945 domain-containing protein -
I3O_RS06525 1254985..1255470 + 486 WP_000214398.1 antirestriction protein -
I3O_RS06530 1255486..1255962 + 477 WP_001345397.1 RadC family protein -
I3O_RS06535 1256025..1256246 + 222 WP_001220314.1 DUF987 domain-containing protein -
I3O_RS06540 1256409..1256783 + 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
I3O_RS06545 1256830..1257204 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
I3O_RS06550 1257201..1257692 + 492 WP_000976857.1 hypothetical protein -
I3O_RS06555 1257704..1257901 + 198 WP_000839282.1 DUF957 domain-containing protein -
I3O_RS06560 1257986..1258828 + 843 WP_001280481.1 DUF4942 domain-containing protein -
I3O_RS06570 1259299..1260237 + 939 WP_000351317.1 glyoxylate/hydroxypyruvate reductase GhrA -
I3O_RS06575 1260292..1261029 + 738 WP_000283664.1 zinc-binding phosphatase -
I3O_RS06580 1261053..1261607 + 555 WP_001001902.1 molecular chaperone YcdY -
I3O_RS06585 1261709..1262200 + 492 WP_001297187.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 1255486..1267765 12279


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T100560 WP_000854914.1 NZ_CP028117:1256830-1257204 [Escherichia coli O111 str. RM9322]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T100560 NZ_CP028117:1256830-1257204 [Escherichia coli O111 str. RM9322]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT100560 WP_001280954.1 NZ_CP028117:1256409-1256783 [Escherichia coli O111 str. RM9322]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT100560 NZ_CP028117:1256409-1256783 [Escherichia coli O111 str. RM9322]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References